Cytochrome b fusion to formaldehyde dehydrogenase enables increased direct electron transfer
Drommershausen, A.-L.; Schnell, B. H. G.; Holtmann, D.
2026. Journal of Electroanalytical Chemistry, 1010, 120044. doi:10.1016/j.jelechem.2026.120044

Adaptive Laboratory Evolution of Cupriavidus necator to Improve Energy Demand in Bioelectrochemical Cultivations
van der Sande, L.; Müller, M.; Holtmann, D.
2026. ChemElectroChem, 13 (5). doi:10.1002/celc.70166

Magnetic actuation of fungal pellets via immobilization of ferromagnetic particles
Hartmann, L.; Schreidl, M.; Pyschik, M.; Stöckl, M.; Holtmann, D.
2026. Chemical Engineering and Processing - Process Intensification, 222, 110750. doi:10.1016/j.cep.2026.110750

Influence of Zn and Oxygen Supply on Malic Acid Production and Growth of Aspergillus oryzae
Hartmann, L.; Neumann, A.; Holtmann, D.; Ochsenreither, K.
2026. Biotechnology and Bioengineering. doi:10.1002/bit.70160

Ressourceneffiziente Kreislaufsysteme am KIT (REKS@KIT): Herausforderungen – Forschungsbedarfe – Empfehlungen
Albiez, M.; Dehn, F.; Eiche, E.; Grauberger, P.; Hilgers, C.; Holtmann, D.; Hoose, C.; Kaster, A.-K.; Kirchlechner, C.; Meier, M.; Rabe, K.; Matthiesen, S.; Schultmann, F.; Schulze, V.; Selzer, M.; Stapf, D.; Stemmermann, P.; Stutz, H.-H.; Ummenhofer, T.; Volk, R.; Wilcke, W.
2026. (C. Hilgers & V. Schulze, Eds.), Karlsruher Institut für Technologie (KIT). doi:10.5445/IR/1000189474

Extracellular Bacterial Production of DNA Hydrogels–Toward Engineered Living Materials
Gaspers, P.; Bickmann, C.; Wallner, C.; Diaz, D. B.; Holtmann, D.; Gescher, J.; Rabe, K. S.; Niemeyer, C. M.
2025. Small, 21 (19), Art.-Nr.: 2502199. doi:10.1002/smll.202502199

On-line monitoring of electroactive biofilms on the electrodes of bioelectrochemical systems by means of heat transfer biofilm sensors. PhD dissertation
Netsch, A.
2025, November 27. Karlsruher Institut für Technologie (KIT). doi:10.5445/IR/1000187568

Exploitation of Biodiversity in Bioeconomy: Examples, Opportunities, and Challenges
Grünberger, A.; Schepp, E.; Lang, S.; Schulz, K. E.; Diaz, D. B.; Sivanesapillai, A.; Diepold, A.; Stolle, C.; Rabe, K. S.; Ulber, R.; Holtmann, D.
2025. Advances in Biochemical Engineering/Biotechnology, 1–25, Springer Nature Switzerland. doi:10.1007/10_2025_288

Holistic Evaluation of Enzyme Immobilization Processes: A Method for Evaluating the Entire Production Process
Teetz, N.; Drommershausen, A.-L.; Gebele, L.; Holtmann, D.
2025. ChemCatChem, 17 (14). doi:10.1002/cctc.202500699

Understanding the Role of pH Regulation and Neutralizing Agents in Organic Acid Production and Growth of Aspergillus oryzae
Hartmann, L.; Martin, M. C.; Neumann, A.; Holtmann, D.; Ochsenreither, K.
2026. Biotechnology and Bioengineering, 123 (1), 116–133. doi:10.1002/bit.70091

Low Oxygen Availability Increases Itaconate Production by Ustilago maydis
Volkmar, M.; Laudensack, W.; Bartzack, F.; Erdmann, N.; Schönrock, S.; Fuderer, E.; Holtmann, D.; Blank, L. M.; Ulber, R.
2025. Biotechnology and Bioengineering, 122 (11), 3007–3017. doi:10.1002/bit.70035

Integrated co-cultivation and subsequent esterification: Harnessing Saccharomyces cerevisiae and Clostridium tyrobutyricum for streamlined ester production
Oehlenschläger, K.; Lorenz, M.; Schepp, E.; Di Nonno, S.; Holtmann, D.; Ulber, R.
2025. Biotechnology for Biofuels and Bioproducts, 18 (1), Art.-Nr.: 98. doi:10.1186/s13068-025-02698-3

Optimizing Yeast Surface-Displayed Unspecific Peroxygenase Production for Sustainable Biocatalysis
Teetz, N.; Zuhse, L.; Holtmann, D.
2025. Bioengineering, 12 (8), 822. doi:10.3390/bioengineering12080822

Process Development for Hepatitis B core Antigen Virus-Like Particles as Gene Delivery Vectors - Processing and Analytics in the Presence of Nucleic Acids. PhD dissertation
Valentic, A.
2025, July 17. Karlsruher Institut für Technologie (KIT). doi:10.5445/IR/1000183128

Robustness of the Cupriavidus necator-Catalyzed Production of α-Humulene
Becker, L.; Dietz, E.; Holtmann, D.
2025. Bioengineering, 12 (3), Art.-Nr.: 323. doi:10.3390/bioengineering12030323

A co-feeding strategy of formate and H for methanogens – Enhancing growth parameters and methane production
Sabel-Becker, B.; Jost, N. P.; Kaster, A.-K.; Holtmann, D.
2025. Journal of CO2 Utilization, 93, 103049. doi:10.1016/j.jcou.2025.103049

Individual process steps optimization of Cupriavidus necator-catalyzed production of α-humulene
Becker, L.; Dietz, E.; Holtmann, D.
2025. Biochemical Engineering Journal, 215, 109617. doi:10.1016/j.bej.2024.109617

Electrodes from carbonized grass clippings for bioelectrochemical systems
Langsdorf, A.; Halim, M.; Volkmar, M.; Stöckl, M.; Harnisch, R.; Hahn, P.; Ulber, R.; Holtmann, D.
2024. Cleaner Chemical Engineering, 9, 100118. doi:10.1016/j.clce.2024.100118

Value adding bioconversion of residues and byproducts—a logistics challenge
Rudi, A.; Schönrock, S.; Laudensack, W.; Schultmann, F.; Ulber, R.; Holtmann, D.
2024. Discover Applied Sciences, 6 (11), Art.-Nr.: 592. doi:10.1007/s42452-024-06286-2

Combining biocatalytic oxyfunctionalisation and organocatalytic aldol reaction to access chiral β-hydroxy ketones
Wang, Y.; Domestici, C.; Teetz, N.; Holtmann, D.; Alcalde, M.; Wang, M.; Qi, W.; Zhang, W.; Hollmann, F.
2024. Molecular Catalysis, 569, 114515. doi:10.1016/j.mcat.2024.114515

Yeast Surface Display Enables One‐Step Production and Immobilization of Unspecific Peroxygenases
Teetz, N.; Lang, S.; Liese, A.; Holtmann, D.
2024. ChemCatChem, 16 (21), e202400908. doi:10.1002/cctc.202400908

Unraveling the Electron Transfer in Cupriavidus necator – Insights Into Mediator Reduction Mechanics
Gemünde, A.; Ruppert, N.-L.; Holtmann, D.
2024. ChemElectroChem, 11 (14), Art.-Nr.: e202400273. doi:10.1002/celc.202400273

Simultaneous fermentation and enzymatic biocatalysis—a useful process option?
Oehlenschläger, K.; Schepp, E.; Stiefelmaier, J.; Holtmann, D.; Ulber, R.
2024. Biotechnology for Biofuels and Bioproducts, 17 (1), Art.-Nr.: 67. doi:10.1186/s13068-024-02519-z

Production of polyhydroxybutyrate from industrial flue gas by microbial electrosynthesis
Langsdorf, A.; Schütz, J. P.; Ulber, R.; Stöckl, M.; Holtmann, D.
2024. Journal of CO2 Utilization, 83, Art.-Nr.: 102800. doi:10.1016/j.jcou.2024.102800

Redox mediator interaction with Cupriavidus necator – spectroelectrochemical online analysis
Gemünde, A.; Gail, J.; Holtmann, D.
2024. Electrochemistry Communications, 162, Art.-Nr.: 107705. doi:10.1016/j.elecom.2024.107705

Microbial electrotechnology – Intensification of bioprocesses through the combination of electrochemistry and biotechnology
Stöckl, M.; Gemünde, A.; Holtmann, D.
2024. Physical sciences reviews, 9 (8), 2775–2793. doi:10.1515/psr-2022-0108

Chemoorganotrophic electrofermentation by Cupriavidus necator using redox mediators
Gemünde, A.; Rossini, E.; Lenz, O.; Frielingsdorf, S.; Holtmann, D.
2024. Bioelectrochemistry, 158, Art.-Nr.: 108694. doi:10.1016/j.bioelechem.2024.108694

Intensification of bioprocesses – definition, examples, challenges and future directions
Hartmann, L.; Krieg, T.; Holtmann, D.
2024. Physical Sciences Reviews, 9 (10), 3273–3287. doi:10.1515/psr-2022-0101

Anti-inflammatory effects of α-humulene on the release of pro-inflammatory cytokines in lipopolysaccharide-induced THP-1 cells
Becker, L.; Holtmann, D.
2024. Cell Biochemistry and Biophysics, 82 (2), 839–847. doi:10.1007/s12013-024-01235-7

Coupling of CO Electrolysis with Parallel and Semi‐Automated Biopolymer Synthesis – Ex‐Cell and without Downstream Processing
Dinges, I.; Depentori, I.; Gans, L.; Holtmann, D.; Waldvogel, S. R.; Stöckl, M.
2024. ChemSusChem, Art.-Nr.: e202301721. doi:10.1002/cssc.202301721

New insights into the influence of pre-culture on robust solvent production of C. acetobutylicum
Oehlenschläger, K.; Volkmar, M.; Stiefelmaier, J.; Langsdorf, A.; Holtmann, D.; Tippkötter, N.; Ulber, R.
2024. Applied Microbiology and Biotechnology, 108 (1), Art.-Nr.: 143. doi:10.1007/s00253-023-12981-8

Metabolic engineering of Shewanella oneidensis to produce glutamate and itaconic acid
Wohlers, H.; Zentgraf, L.; van der Sande, L.; Holtmann, D.
2024. Applied Microbiology and Biotechnology, 108 (1). doi:10.1007/s00253-023-12879-5

Autotrophic Production of the Sesquiterpene α-Humulene with Cupriavidus necator in a Controlled Bioreactor
Sydow, A.; Becker, L.; Lombard, E.; Ulber, R.; Guillouet, S. E.; Holtmann, D.
2023. Bioengineering, 10 (10), 1194. doi:10.3390/bioengineering10101194

Electrochemical HO - stat mode as reaction concept to improve the process performance of an unspecific peroxygenase
Sayoga, G. V.; Bueschler, V. S.; Beisch, H.; Utesch, T.; Holtmann, D.; Fiedler, B.; Ohde, D.; Liese, A.
2023. New Biotechnology, 78, 95 – 104. doi:10.1016/j.nbt.2023.10.007

Scalable Unseparated Bioelectrochemical Reactors by Using a Carbon Fiber Brush as Stirrer and Working Electrode
Hengsbach, J.-N.; Engel, M.; Cwienczek, M.; Stiefelmaier, J.; Tippkötter, N.; Holtmann, D.; Ulber, R.
2023. ChemElectroChem, 10 (21), Art.-Nr.: e202300440. doi:10.1002/celc.202300440

Quantitative and Non‐Quantitative Assessments of Enzymatic Electrosynthesis: A Case Study of Parameter Requirements
Sayoga, G.; Abt, M.; Teetz, N.; Bueschler, V.; Liese, A.; Franzreb, M.; Holtmann, D.
2023. ChemElectroChem, 10 (24), Art.Nr.: e202300226. doi:10.1002/celc.202300226

Municipal green waste as substrate for the microbial production of platform chemicals
Volkmar, M.; Maus, A.-L.; Weisbrodt, M.; Bohlender, J.; Langsdorf, A.; Holtmann, D.; Ulber, R.
2023. Bioresources and Bioprocessing, 10 (1), Art.-Nr.: 43. doi:10.1186/s40643-023-00663-2

Antibiofilm assay for antimicrobial peptides combating the sulfate‐reducing bacteria Desulfovibrio vulgaris
Stillger, L.; Viau, L.; Holtmann, D.; Müller, D.
2023. MicrobiologyOpen, 12 (4), Art.-Nr.: e1376. doi:10.1002/mbo3.1376

Process intensification using immobilized enzymes
Bolat, S.; Greifenstein, R.; Franzreb, M.; Holtmann, D.
2023. Physical Sciences Reviews. doi:10.1515/psr-2022-0110

Application of the all-in-one electrode for in situ HO generation in hydroxylation catalyzed by unspecific peroxygenase from Agrocybe aegerita
Sayoga, G. V.; Bueschler, V. S.; Beisch, H.; Holtmann, D.; Zeng, A.-P.; Fiedler, B.; Ohde, D.; Liese, A.
2023. Molecular Catalysis, 547, Art.-Nr.: 113325. doi:10.1016/j.mcat.2023.113325

Contribution of Enzyme Catalysis to the Achievement of the United Nations’ Sustainable Development Goals
Holtmann, D.; Hollmann, F.; Bouchaut, B.
2023. Molecules, 28 (10), Art.-Nr.: 4125. doi:10.3390/molecules28104125

Deep Eutectic Solvents as Efficient Solvents in Biocatalysis
Pätzold, M.; Siebenhaller, S.; Kara, S.; Liese, A.; Syldatk, C.; Holtmann, D.
2019. Trends in biotechnology, 37 (9), 943–959. doi:10.1016/j.tibtech.2019.03.007

Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms
Arinda, T.; Philipp, L.-A.; Rehnlund, D.; Edel, M.; Chodorski, J.; Stöckl, M.; Holtmann, D.; Ulber, R.; Gescher, J.; Sturm-Richter, K.
2019. Frontiers in microbiology, 10, Article: 126. doi:10.3389/fmicb.2019.00126

DSP of biomolecules
Franzreb, M.; Holtmann, D.
2014. Kreysa, G. [Hrsg.] Encyclopedia of Applied Electrochemistry New York, N.Y. : Springer Science+Business Media, 2014, 351–357