Dr. Jens Rudat Tebi_Dr. Jens Rudat

PD Dr. (Dipl.-Biol.) Jens Rudat

  • Lecturer
  • Lectures BING- Biochemistry, and Industrial Biocatalysis; microscopy instruction
  • group: 

    Biokatalyse

  • office hours: 

    by arrangement

  • room: 118 in 30.44, 1. OG
  • phone: +49 721 608-48428

  • Fritz-Haber-Weg 4, 76131 Karlsruhe

Curriculum vitae

Academic Career

Since February 2022 Privatdozent, with the right to teach "Technical Biology"
Since November 2012 Akademischer Rat (bleibt) at KIT-CS, Institute of Process Engineering in Life Sciences, Division II: Technical Biology
2006–2012 Group Leader of “Biocatalysis” at KIT-CS (until 2009, University of Karlsruhe), IBLT_II: Technical Biology
2001–2006 Doctorate at the Institute of Microbiology & Biotechnology at the University of Bonn under the supervision of PD Dr. René M. Fakoussa: Enzymatic decarboxylation of benzene polycarboxylic acids
1995–2000 Studied biology (Diploma) at the Rheinische Friedrich-Wilhelms-Universität Bonn

Awards

3rd prize in the NEULAND_2022 innovation competition in the “Ideas Prize” category for the BioHealing project—a self-healing fuel cell

Award for Excellence in Teaching from the Department of Chemical and Process Engineering at KIT (2012)

Recipient of a doctoral fellowship from the State of North Rhine-Westphalia (2001–2003)

 

Research Interests

Biotransformation of nitrogen heterocycles (hydantoins, dihydropyrimidines, diketopiperazines) as well as canonical and non-canonical amino acids, including for semisynthetic penicillins.
Bacterial metabolism of aromatic compounds and nitrogen.

Use of renewable raw materials for the synthesis of fine chemicals.

Molecular biological and process engineering optimization of enzymatic reactions: genetic engineering, heterologous (over)expression, various immobilization methods.
 

International research collaborations

  • 2008–2012 and 2016–2017: Member of the European COST program in the research networks “CASCAT – Cascade Chemo-Enzymatic Process” and “Systems Biocatalysis.” Participation in two COST summer schools and numerous international workshops by five doctoral candidates from the group.
  • 2010–2015: Participation in two research collaborations with South Africa (University of Cape Town and University of Western Cape); this included research stays of several months by seven members of the department’s staff there, as well as reciprocal visits by South African scientists.
  • Since the 2016–2017 winter semester, project leader of a collaboration with the China Scholarship Council, including supervision of a Chinese doctoral candidate in the LigArom project
  • Winter semester 2018/19: Organized and supervised a research stay for an Iranian visiting scholar

 

Other

Serves as a reviewer for the journals *Nature Communications*, *Applied Microbiology and Biotechnology*, *Biocatalysis and Biotransformation*, *Journal of Biotechnology*, *Molecules*, *Enzyme and Microbial Technology*, *Applied Biochemistry and Biotechnology*, *International Journal of Molecular Sciences*, and *AMB Express*.
Membership in the Association for General and Applied Microbiology (VAAM) and the DECHEMA Society for Chemical Engineering and Biotechnology; member of the Biotransformation and Space Microbiology divisions.

 

Teaching Activities

Introductory lecture on “Biochemistry,” block seminar on “Presentation Skills,” and major-specific lecture on “Industrial Biocatalysis” for the degree programs in Bioengineering, Chemical Engineering, Biology, and Bioeconomics; Authorized to grade theses in the aforementioned degree programs as well as in the Teacher Education program in Natural Sciences and Technology (NwT).

Public lectures, e.g., Karlsruhe Night of Science, Stuttgart Science Festival, Academy for Scientific Continued Education (AWWK), Science and Music, Science Slam (1st place at Microbe Slam 2018), KIT Children’s University

Kinder-Uni 

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IdeenExpo 

 

Publications


The Promising Role of Amine Transaminase Cascades in the Synthesis of Non-Canonical Amino Acids
Gord Noshahri, N.; Rudat, J.
2024. Processes, 12 (11), 2566. doi:10.3390/pr12112566
Enzyme Immobilization on Polypropylene Film: A Role Model for Biocatalytic Polymer Membranes?
Gartner, P.; Rudat, J.; Bilger, M.; Gruenert, T.; Lanza, G.
2023. Journal of Enzymes, 1 (3), 1–12. doi:10.14302/issn.2690-4829.jen-23-4799
Development of two devices for high-throughput screening of ethanol-producing microorganisms by real-time CO₂ production monitoring
Gord Noshahri, N.; Sharifi, A.; Seyedabadi, M.; Rudat, J.; Zare Mehrjerdi, M.
2023. Bioprocess and Biosystems Engineering, 46 (8), 1209–1220. doi:10.1007/s00449-023-02892-3
Self-healing Fuel Cells by Biological Actuators
Gartner, P.; Lanza, G.; Rudat, J.; Bilger, M.; Grünert, T.; Nesterov-Mueller, A.; Zimmerer, N.; Quarz, P.; Scharfer, P.; Schabel, W.; Jung, A. P.; Stahlberger, M.; Bräse, S.
2023. Procedia CIRP, 116, 161–166. doi:10.1016/j.procir.2023.02.028
Selective Peptide Binders to the Perfluorinated Sulfonic Acid Ionomer Nafion
Schmidt, D.; Gartner, P.; Berezkin, I.; Rudat, J.; Bilger, M.; Grünert, T.; Zimmerer, N.; Quarz, P.; Scharfer, P.; Brückel, J.; Jung, A. P.; Singh, P.; Pooja, P.; Meier, B.; Stahlberger, M.; Schabel, W.; Bräse, S.; Lanza, G.; Nesterov-Mueller, A.
2024. Advanced Functional Materials, 34 (20), Article no: 2214932. doi:10.1002/adfm.202214932
Enhanced Bioactivity of Tailor-Made Glycolipid Enriched Manuka Honey
Delavault, A.; Zoheir, A. E.; Muller, D.; Hollenbach, R.; Rabe, K. S.; Ochsenreither, K.; Rudat, J.; Syldatk, C.
2022. International Journal of Molecular Sciences, 23 (19), Art.-Nr.: 12031. doi:10.3390/ijms231912031
Origin and Evolution of Enzymes with MIO Prosthetic Group: Microbial Coevolution After the Mass Extinction Event
Peng, F.; Engel, U.; Aliyu, H.; Rudat, J.
2022. Frontiers in Genetics, 13, Art.-Nr. 851738. doi:10.3389/fgene.2022.851738
Synthesis of (S)- and (R)-β-Tyrosine by Redesigned Phenylalanine Aminomutase
Peng, F.; Aliyu, H.; Delavault, A.; Engel, U.; Rudat, J.
2022. Catalysts, 12 (4), Art.-Nr.: 397. doi:10.3390/catal12040397
Production of Lignin-Based Aromatic Amino Acids with Computational Designed Phenylalanine Aminomutase from . PhD dissertation
Peng, F.
2022, May 3. Karlsruher Institut für Technologie (KIT). doi:10.5445/IR/1000145124
Alanins Wunderlampe : Vorkommen, Nutzung und Produktion nicht-kanonischer Aminosäuren
Rudat, J.; Engel, U.
2021. Biologie in unserer Zeit, 51 (4), 376–386. doi:10.11576/ biuz-4827
Enzymatic Synthesis of Alkyl Glucosides by β‐Glucosidases in a 2‐in‐1 Deep Eutectic Solvent System 
Delavault, A.; Grüninger, J.; Kapp, D.; Hollenbach, R.; Rudat, J.; Ochsenreither, K.; Syldatk, C.
2022. Chemie - Ingenieur - Technik, 94 (3), 417–426. doi:10.1002/cite.202100150
Computational-designed enzyme for β-tyrosine production in lignin valorization
Peng, F.; Aliyu, H.; Delavault, A.; Engel, U.; Rudat, J.
2021. Catalysts, 11 (11), 1310. doi:10.3390/catal11111310
Comparative study on interfacial and foaming properties of glycolipids in relation to the gas applied for foam generation
Hollenbach, R.; Oeppling, S.; Delavault, A.; Völp, A. R.; Willenbacher, N.; Rudat, J.; Ochsenreither, K.; Syldatk, C.
2021. RSC Advances, 11 (54), 34235–34244. doi:10.1039/D1RA06190A
Growth optimization and identification of an ω-transaminase by a novel native PAGE activity staining method in a Bacillus sp. strain BaH isolated from Iranian soil
Gord Noshahri, N.; Fooladi, J.; Engel, U.; Muller, D.; Kugel, M.; Gorenflo, P.; Syldatk, C.; Rudat, J.
2021. AMB Express, 11 (1), Art.-Nr.: 46. doi:10.1186/s13568-021-01207-7
Interfacial and Foaming Properties of Tailor-Made Glycolipids—Influence of the Hydrophilic Head Group and Functional Groups in the Hydrophobic Tail
Hollenbach, R.; Völp, A. R.; Höfert, L.; Rudat, J.; Ochsenreither, K.; Willenbacher, N.; Syldatk, C.
2020. Molecules, 25 (17), 3797. doi:10.3390/molecules25173797
Screening and comparative characterization of microorganisms from Iranian soil samples showing ω-transaminase activity toward a plethora of substrates
Noshahri, N. G.; Fooladi, J.; Syldatk, C.; Engel, U.; Heravi, M. M.; Mehrjerdi, M. Z.; Rudat, J.
2019. Catalysts, 9 (10), Article No.874. doi:10.3390/catal9100874
Enantiomer discrimination in β-phenylalanine degradation by a newly isolated Paraburkholderia strain BS115 and type strain PsJN
Buß, O.; Dold, S.-M.; Obermeier, P.; Litty, D.; Muller, D.; Grüninger, J.; Rudat, J.
2018. AMB express, 8 (1), Article: 149. doi:10.1186/s13568-018-0676-2
β-Phenylalanine Ester Synthesis from Stable β-Keto Ester Substrate Using Engineered ω-Transaminases
Buß, O.; Voss, M.; Delavault, A.; Gorenflo, P.; Syldatk, C.; Bornscheuer, U.; Rudat, J.
2018. Molecules, 23 (5), Article: 1211. doi:10.3390/molecules23051211
The ω-transaminase engineering database (oTAED) : A navigation tool in protein sequence and structure space
Buß, O.; Buchholz, P. C. F.; Gräff, M.; Klausmann, P.; Rudat, J.; Pleiss, J.
2018. Proteins, 86 (5), 566–580. doi:10.1002/prot.25477
FoldX as Protein Engineering Tool: Better Than Random Based Approaches?
Buß, O.; Rudat, J.; Ochsenreither, K.
2018. Computational and structural biotechnology journal, 16, 25–33. doi:10.1016/j.csbj.2018.01.002
Improvement in the Thermostability of a β-Amino Acid Converting ω-Transaminase by Using FoldX
Buß, O.; Muller, D.; Jager, S.; Rudat, J.; Rabe, K. S.
2018. ChemBioChem, 19 (4), 379–387. doi:10.1002/cbic.201700467
Toward a cell-free hydantoinase process : screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase
Slomka, C.; Späth, G. P.; Lemke, P.; Skoupi, M.; Niemeyer, C. M.; Syldatk, C.; Rudat, J.
2017. AMB express, 7 (1), Art. Nr. 122. doi:10.1186/s13568-017-0420-3
Phenylalanine ammonia lyase from Arabidopsis thaliana (AtPAL2) : A potent MIO-enzyme for the synthesis of non-canonical aromatic alpha-amino acids. Part I: Comparative characterization to the enzymes from Petroselinum crispum (PcPAL1) and Rhodosporidium toruloides (RtPAL)
Dreßen, A.; Hilberath, T.; Mackfeld, U.; Billmeier, A.; Rudat, J.; Pohl, M.
2017. Journal of biotechnology, 258, 148–157. doi:10.1016/j.jbiotec.2017.04.005
Phenylalanine ammonia lyase from Arabidopsis thaliana (AtPAL2) : A potent MIO-enzyme for the synthesis of non-canonical aromatic alpha-amino acids.. Part II: Application in different reactor concepts for the production of (S)-2-chloro-phenylalanine
Dreßen, A.; Hilberath, T.; Mackfeld, U.; Rudat, J.; Pohl, M.
2017. Journal of biotechnology, 258, 158–166. doi:10.1016/j.jbiotec.2017.04.035
Hydantoinasen – von der präbiotischen Evolution zur Aminosäureproduktion
Rudat, J.; Engel, U.
2017. Biospektrum, 23 (1), 98–100. doi:10.1007/s12268-017-0770-z
Transaminases and their applications
Dold, S.-M.; Syldatk, C.; Rudat, J.
2016. Green biocatalysis. Ed.: R. Patel, 715–746, Wiley-VCH Verlag. doi:10.1002/9781118828083.ch29
One-step purification and immobilization of a β-amino acid aminotransferase using magnetic (M-PVA) beads
Dold, S.-M.; Cai, L.; Rudat, J.
2016. Engineering in life sciences / Special Issue: Molecular Interaction Engineering, 16 (6), 568–576. doi:10.1002/elsc.201600042
Stereoselective synthesis of β-amino acids by hydrolysis of an aryl-substituted dihydropyrimidine by hydantoinases
Engel, U.; Syldatk, C.; Rudat, J.
2016. Practical Methods for Biocatalysis and Biotransformations 3. Ed.: J. Whittall, John Wiley and Sons
Statistical Evaluation of HTS Assays for Enzymatic Hydrolysis of β-Keto Esters
Buß, O.; Jager, S.; Dold, S. M.; Zimmermann, S.; Hamacher, K.; Schmitz, K.; Rudat, J.
2016. PLoS one, 11 (1), e0146104. doi:10.1371/journal.pone.0146104
Chemical synthesis and enzymatic, stereoselective hydrolysis of a functionalized dihydropyrimidine for the synthesis of β-amino acids
Slomka, C.; Zhong, S.; Fellinger, A.; Engel, U.; Syldatk, C.; Bräse, S.; Rudat, J.
2015. AMB Express, 5 (1), 85. doi:10.1186/s13568-015-0174-8
Hydrolysis of Hydantoins, Dihydropyrimidines, and Related Compounds
Slomka, C.; Engel, U.; Syldatk, C.; Rudat, J.
2015. Biocatalysis in Organic Synthesis 1. Ed.: K. Faber, 373–414, Thieme Gruppe
The Hydantoinase Process: Recent developments for the production of non-canonical amino acids
Engel, U.; Rudat, J.; Syldatk, C.
2014. Industrial biocatalysis. Ed.: P. Grunwald, Chapter 22, 817–862, Pan Stanford Publ
Cationic heterooligopeptides by ficain-catalyzed co-oligomerization of lysine and methionine ethylesters
Andre, M.; Kühl, B.; Brenner-Weiss, G.; Syldatk, C.; Rudat, J.
2014. Journal of peptide science, 20, 625–629. doi:10.1002/psc.2639
Enzymatical and microbial degradation of cyclic dipeptides (diketopiperazines)
Perzborn, M.; Syldatk, C.; Rudat, J.
2013. AMB Express, 3 (1), 12 S. doi:10.1186/2191-0855-3-51
Separation of Cyclic Dipeptides (Diketopiperazines) from Their Corresponding Linear Dipeptides by RP-HPLC and Method Validation
Perzborn, M.; Syldatk, C.; Rudat, J.
2013. Chromatography research international, 2013, 310269. doi:10.1155/2013/310269
Synthesis of aromatic beta-amino-acid with new cyclic amidases
Bretschneider, U.; Syldatk, C.; Rudat, J.
2010. Chemie Ingenieur Technik, 82 (1-2), 161–165
Microbial conversion of B-Phenyl-alanine using new transaminase
Brucher, B.; Syldatk, C.; Rudat, J.
2010. Chemie Ingenieur Technik, 82, 155–160
Enantioseparation of Aromatic beta(A3)-Amino acid by Precolumn Derivatization with o-Phthaldialdehyde and N-Isobutyryl-l-cysteine
Brucher, B.; Rudat, J.; Syldatk, C.; Vielhauer, O.
2010. Chromatographia, 71 (11-12), 1063–1067. doi:10.1365/s10337-010-1578-x
Food safety: Heat inactivation of Cronobacter in powdered infant formula
Baumann, S.; Rudat, J.
2011. International Journal of Medical Microbiology, 301, 59/1–1
Transaminases for the synthesis of enantiopure beta-amino acids
Rudat, J.; Brucher, B.; Syldatk, C.
2012. AMB Express, 2 (1), 11. doi:10.1186/2191-0855-2-11
Stereoselective hydrolysis of aryl-substituted dihydropyrimidines by hydantoinases
Engel, U.; Syldatk, C.; Rudat, J.
2012. Applied microbiology and biotechnology, 94 (5), 1221–1231. doi:10.1007/s00253-011-3691-7
Novel amidases of two Aminobacter sp. strains: Biotransformation experiments and elucidation of gene sequences
Engel, U.; Syldatk, C.; Rudat, J.
2012. AMB express, 2 (1), 33. doi:10.1186/2191-0855-2-33